Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 499582..500218 Replicon chromosome
Accession NZ_CP120577
Organism Bacillus subtilis strain PRO53

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag P5628_RS02550 Protein ID WP_003156187.1
Coordinates 499868..500218 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag P5628_RS02545 Protein ID WP_003225183.1
Coordinates 499582..499863 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5628_RS02525 (P5628_02525) 495942..496541 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
P5628_RS02530 (P5628_02530) 496636..497001 + 366 WP_003234281.1 holo-ACP synthase -
P5628_RS02535 (P5628_02535) 497167..498183 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
P5628_RS02540 (P5628_02540) 498297..499466 + 1170 WP_015252766.1 alanine racemase -
P5628_RS02545 (P5628_02545) 499582..499863 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
P5628_RS02550 (P5628_02550) 499868..500218 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
P5628_RS02555 (P5628_02555) 500334..501158 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
P5628_RS02560 (P5628_02560) 501163..501528 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
P5628_RS02565 (P5628_02565) 501532..501933 + 402 WP_003225192.1 serine/threonine-protein kinase RsbT -
P5628_RS02570 (P5628_02570) 501945..502952 + 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
P5628_RS02575 (P5628_02575) 503021..503350 + 330 WP_014475877.1 anti-sigma factor antagonist RsbV -
P5628_RS02580 (P5628_02580) 503347..503829 + 483 WP_021481702.1 anti-sigma B factor RsbW -
P5628_RS02585 (P5628_02585) 503795..504583 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
P5628_RS02590 (P5628_02590) 504583..505182 + 600 WP_021481701.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T275230 WP_003156187.1 NZ_CP120577:499868-500218 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT275230 WP_003225183.1 NZ_CP120577:499582-499863 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References