Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3224505..3225159 Replicon chromosome
Accession NC_017651
Organism Escherichia coli str. clone D i2

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag I02_RS15810 Protein ID WP_000244781.1
Coordinates 3224505..3224912 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I02_RS15815 Protein ID WP_000354046.1
Coordinates 3224893..3225159 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I02_RS15790 3220462..3222195 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
I02_RS15795 3222201..3222911 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I02_RS15800 3222936..3223832 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I02_RS15805 3223944..3224465 + 522 WP_001055874.1 flavodoxin FldB -
I02_RS15810 3224505..3224912 - 408 WP_000244781.1 protein YgfX Toxin
I02_RS15815 3224893..3225159 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I02_RS15820 3225402..3226382 + 981 WP_000886079.1 tRNA-modifying protein YgfZ -
I02_RS15825 3226459..3227118 - 660 WP_000250274.1 hemolysin III family protein -
I02_RS15830 3227282..3227593 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
I02_RS15835 3227638..3229071 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T27523 WP_000244781.1 NC_017651:c3224912-3224505 [Escherichia coli str. 'clone D i2']
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T27523 NC_017651:c3224912-3224505 [Escherichia coli str. 'clone D i2']
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT27523 WP_000354046.1 NC_017651:c3225159-3224893 [Escherichia coli str. 'clone D i2']
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT27523 NC_017651:c3225159-3224893 [Escherichia coli str. 'clone D i2']
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTTTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References