Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4399665..4400162 Replicon chromosome
Accession NZ_CP120568
Organism Escherichia coli strain 582

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag P5711_RS21655 Protein ID WP_001173343.1
Coordinates 4399665..4399838 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag P5711_RS21660 Protein ID WP_000398619.1
Coordinates 4399866..4400162 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5711_RS21645 (4396335) 4396335..4397792 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
P5711_RS21650 (4398029) 4398029..4399546 + 1518 WP_001295074.1 lysine--tRNA ligase -
P5711_RS21655 (4399665) 4399665..4399838 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
P5711_RS21660 (4399866) 4399866..4400162 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
P5711_RS21665 (4400389) 4400389..4400661 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
P5711_RS21670 (4400673) 4400673..4400903 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
P5711_RS21675 (4401084) 4401084..4402715 + 1632 WP_001216477.1 sensor histidine kinase -
P5711_RS21680 (4402712) 4402712..4403431 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T275221 WP_001173343.1 NZ_CP120568:c4399838-4399665 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT275221 WP_000398619.1 NZ_CP120568:c4400162-4399866 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References