Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2305175..2306006 Replicon chromosome
Accession NC_017651
Organism Escherichia coli str. clone D i2

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag I02_RS11630 Protein ID WP_000854814.1
Coordinates 2305632..2306006 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag I02_RS11625 Protein ID WP_001285586.1
Coordinates 2305175..2305543 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I02_RS11590 2300392..2301462 + 1071 WP_000102631.1 patatin-like phospholipase family protein -
I02_RS11595 2301598..2302275 + 678 WP_001362823.1 hypothetical protein -
I02_RS11600 2302291..2302701 + 411 WP_000846704.1 hypothetical protein -
I02_RS11605 2302922..2303743 + 822 WP_001234569.1 DUF945 domain-containing protein -
I02_RS11610 2303834..2304319 + 486 WP_000213703.1 antirestriction protein -
I02_RS11615 2304335..2304811 + 477 WP_001351157.1 RadC family protein -
I02_RS11620 2304880..2305101 + 222 WP_000692323.1 DUF987 domain-containing protein -
I02_RS11625 2305175..2305543 + 369 WP_001285586.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
I02_RS11630 2305632..2306006 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
I02_RS11635 2306003..2306197 + 195 WP_000988601.1 hypothetical protein -
I02_RS27840 2306210..2306323 + 114 WP_001161660.1 DUF957 domain-containing protein -
I02_RS25995 2306612..2306758 - 147 Protein_2201 transposase domain-containing protein -
I02_RS11645 2306812..2306994 + 183 WP_001016348.1 hypothetical protein -
I02_RS11650 2307095..2307424 - 330 WP_000450409.1 DUF496 family protein -
I02_RS11655 2307596..2308654 - 1059 WP_001200890.1 FUSC family protein -
I02_RS11660 2308852..2309325 - 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
I02_RS11665 2309444..2310610 - 1167 WP_001297905.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T27520 WP_000854814.1 NC_017651:2305632-2306006 [Escherichia coli str. 'clone D i2']
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T27520 NC_017651:2305632-2306006 [Escherichia coli str. 'clone D i2']
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT27520 WP_001285586.1 NC_017651:2305175-2305543 [Escherichia coli str. 'clone D i2']
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT27520 NC_017651:2305175-2305543 [Escherichia coli str. 'clone D i2']
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References