275142

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 4629075..4629912 Replicon chromosome
Accession NZ_CP120557
Organism Escherichia coli strain B771

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag P5709_RS22520 Protein ID WP_000227786.1
Coordinates 4629075..4629617 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag P5709_RS22525 Protein ID WP_001297137.1
Coordinates 4629601..4629912 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5709_RS22500 (4624605) 4624605..4625516 - 912 WP_000705853.1 2-dehydropantoate 2-reductase -
P5709_RS22505 (4625684) 4625684..4626175 + 492 WP_001138904.1 nucleotide binding protein YajQ -
P5709_RS22510 (4626303) 4626303..4627667 - 1365 WP_001000978.1 MFS transporter -
P5709_RS22515 (4628084) 4628084..4629019 + 936 WP_001297127.1 tetratricopeptide repeat protein -
P5709_RS22520 (4629075) 4629075..4629617 - 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
P5709_RS22525 (4629601) 4629601..4629912 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
P5709_RS22530 (4630097) 4630097..4630987 - 891 WP_000971336.1 heme o synthase -
P5709_RS22535 (4630999) 4630999..4631328 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
P5709_RS22540 (4631328) 4631328..4631942 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
P5709_RS22545 (4631932) 4631932..4633923 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
P5709_RS22550 (4633945) 4633945..4634892 - 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T275142 WP_000227786.1 NZ_CP120557:c4629617-4629075 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT275142 WP_001297137.1 NZ_CP120557:c4629912-4629601 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References