Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4251274..4251850 Replicon chromosome
Accession NZ_CP120549
Organism Escherichia coli strain B1172

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag P5710_RS20640 Protein ID WP_001350372.1
Coordinates 4251563..4251850 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0G9FLJ7
Locus tag P5710_RS20635 Protein ID WP_000063155.1
Coordinates 4251274..4251576 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5710_RS20620 (4247802) 4247802..4249952 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
P5710_RS20625 (4250070) 4250070..4250273 + 204 WP_000467859.1 YbdD/YjiX family protein -
P5710_RS20630 (4250284) 4250284..4251240 + 957 WP_001350371.1 GTPase -
P5710_RS20635 (4251274) 4251274..4251576 - 303 WP_000063155.1 BrnA antitoxin family protein Antitoxin
P5710_RS20640 (4251563) 4251563..4251850 - 288 WP_001350372.1 BrnT family toxin Toxin
P5710_RS20645 (4252047) 4252047..4253213 + 1167 WP_000800824.1 restriction endonuclease -
P5710_RS20650 (4253277) 4253277..4254896 + 1620 WP_001029741.1 class I SAM-dependent DNA methyltransferase -
P5710_RS20655 (4254886) 4254886..4256217 + 1332 WP_001560776.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T275112 WP_001350372.1 NZ_CP120549:c4251850-4251563 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11572.05 Da        Isoelectric Point: 9.4646

>AT275112 WP_000063155.1 NZ_CP120549:c4251576-4251274 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0G9FLJ7

References