Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 522235..522871 Replicon chromosome
Accession NZ_CP120511
Organism Bacillus sp. B28

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag P3L57_RS02675 Protein ID WP_003156187.1
Coordinates 522521..522871 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag P3L57_RS02670 Protein ID WP_003225183.1
Coordinates 522235..522516 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P3L57_RS02650 (P3L57_02650) 518592..519191 - 600 WP_024120294.1 rhomboid family intramembrane serine protease -
P3L57_RS02655 (P3L57_02655) 519286..519651 + 366 WP_069487530.1 holo-ACP synthase -
P3L57_RS02660 (P3L57_02660) 519817..520833 + 1017 WP_082687822.1 outer membrane lipoprotein carrier protein LolA -
P3L57_RS02665 (P3L57_02665) 520950..522119 + 1170 WP_044161733.1 alanine racemase -
P3L57_RS02670 (P3L57_02670) 522235..522516 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
P3L57_RS02675 (P3L57_02675) 522521..522871 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
P3L57_RS02680 (P3L57_02680) 522989..523813 + 825 WP_082687807.1 RsbT co-antagonist protein RsbRA -
P3L57_RS02685 (P3L57_02685) 523818..524183 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
P3L57_RS02690 (P3L57_02690) 524187..524588 + 402 WP_024714447.1 serine/threonine-protein kinase RsbT -
P3L57_RS02695 (P3L57_02695) 524600..525607 + 1008 WP_044161721.1 phosphoserine phosphatase RsbU -
P3L57_RS02700 (P3L57_02700) 525668..525997 + 330 WP_024120301.1 anti-sigma factor antagonist RsbV -
P3L57_RS02705 (P3L57_02705) 525994..526476 + 483 WP_024120302.1 anti-sigma B factor RsbW -
P3L57_RS02710 (P3L57_02710) 526442..527230 + 789 WP_024120303.1 RNA polymerase sigma factor SigB -
P3L57_RS02715 (P3L57_02715) 527230..527829 + 600 WP_044161719.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T275083 WP_003156187.1 NZ_CP120511:522521-522871 [Bacillus sp. B28]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT275083 WP_003225183.1 NZ_CP120511:522235-522516 [Bacillus sp. B28]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References