275065

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 42901..44038 Replicon plasmid pBN62-65kb
Accession NZ_CP120418
Organism Lactococcus sp. bn62

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag P3G65_RS11390 Protein ID WP_002332783.1
Coordinates 43175..44038 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag P3G65_RS11385 Protein ID WP_002326825.1
Coordinates 42901..43173 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P3G65_RS11355 39963..40153 + 191 Protein_44 peptide-binding protein -
P3G65_RS11360 40202..40285 + 84 WP_032501549.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
P3G65_RS11365 40410..41147 + 738 WP_001038790.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
P3G65_RS11370 41317..41577 + 261 Protein_47 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
P3G65_RS11375 41680..42576 + 897 WP_002326827.1 ParA family protein -
P3G65_RS11380 42668..42883 + 216 WP_001835296.1 peptide-binding protein -
P3G65_RS11385 42901..43173 + 273 WP_002326825.1 antitoxin Antitoxin
P3G65_RS11390 43175..44038 + 864 WP_002332783.1 zeta toxin family protein Toxin
P3G65_RS11395 44478..44795 + 318 WP_002326830.1 hypothetical protein -
P3G65_RS11400 45092..45589 + 498 WP_000868795.1 trimethoprim-resistant dihydrofolate reductase DfrG -
P3G65_RS11405 45661..46545 - 885 Protein_54 insertion element protein -
P3G65_RS11410 46591..47271 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
P3G65_RS11415 47469..47780 + 312 Protein_56 replication initiation protein -
P3G65_RS11420 47915..48562 + 648 WP_002295562.1 type A chloramphenicol O-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / dfrG / cat - 1..65460 65460
- inside IScluster/Tn erm(B) / dfrG / cat - 36417..49504 13087


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T275065 WP_002332783.1 NZ_CP120418:43175-44038 [Lactococcus sp. bn62]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT275065 WP_002326825.1 NZ_CP120418:42901-43173 [Lactococcus sp. bn62]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References