Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 43691..44216 Replicon plasmid pCE10A
Accession NC_017647
Organism Escherichia coli O7:K1 str. CE10

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CE10_RS27085 Protein ID WP_001159871.1
Coordinates 43911..44216 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag CE10_RS27080 Protein ID WP_000813634.1
Coordinates 43691..43909 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CE10_RS27075 40728..41369 - 642 WP_000990668.1 hypothetical protein -
CE10_RS27080 43691..43909 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CE10_RS27085 43911..44216 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CE10_RS27090 44217..45026 + 810 WP_000016979.1 site-specific integrase -
CE10_RS27095 45164..45439 - 276 WP_000239529.1 hypothetical protein -
CE10_RS27100 45433..46077 - 645 WP_000633911.1 AAA family ATPase -
CE10_RS27105 46306..47277 + 972 WP_001103694.1 hypothetical protein -
CE10_RS27110 47282..47674 + 393 WP_000340835.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - senB 1..54289 54289


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T27503 WP_001159871.1 NC_017647:43911-44216 [Escherichia coli O7:K1 str. CE10]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T27503 NC_017647:43911-44216 [Escherichia coli O7:K1 str. CE10]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT27503 WP_000813634.1 NC_017647:43691-43909 [Escherichia coli O7:K1 str. CE10]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT27503 NC_017647:43691-43909 [Escherichia coli O7:K1 str. CE10]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References