Detailed information of TA system TA027500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 5076610..5077205
• Replicon: chromosome
• Accession: NC_017646
• Organism: Escherichia coli O7:K1 str. CE10

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: CE10_RS25600
• Protein ID: WP_000239581.1
• Coordinates: 5076855..5077205 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: CE10_RS25595
• Protein ID: WP_001223213.1
• Coordinates: 5076610..5076861 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CE10_RS25585	5072275..5076054	+	3780	WP_000060874.1	autotransporter assembly complex protein TamB	-
CE10_RS25590	5076057..5076398	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
CE10_RS25595	5076610..5076861	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
CE10_RS25600	5076855..5077205	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
CE10_RS25605	5077284..5077814	-	531	WP_000055067.1	inorganic diphosphatase	-
CE10_RS25610	5078124..5079080	+	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
CE10_RS25615	5079220..5080722	+	1503	WP_000205813.1	sugar ABC transporter ATP-binding protein	-
CE10_RS25620	5080736..5081758	+	1023	WP_001572298.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T27500 WP_000239581.1 NC_017646:5076855-5077205 [Escherichia coli O7:K1 str. CE10]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T27500 NC_017646:5076855-5077205 [Escherichia coli O7:K1 str. CE10]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGACTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT27500 WP_001223213.1 NC_017646:5076610-5076861 [Escherichia coli O7:K1 str. CE10]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT27500 NC_017646:5076610-5076861 [Escherichia coli O7:K1 str. CE10]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7