Detailed information of TA system TA027498

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4355442..4356276
• Replicon: chromosome
• Accession: NC_017646
• Organism: Escherichia coli O7:K1 str. CE10

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8T5WFL4
• Locus tag: CE10_RS22105
• Protein ID: WP_000854823.1
• Coordinates: 4355899..4356276 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8T5WFE1
• Locus tag: CE10_RS22100
• Protein ID: WP_001285614.1
• Coordinates: 4355442..4355810 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CE10_RS22055	4350734..4351411	+	678	WP_001097562.1	hypothetical protein	-
CE10_RS22060	4351417..4351650	+	234	WP_000883181.1	DUF905 family protein	-
CE10_RS22065	4351740..4352558	+	819	WP_001175143.1	DUF945 domain-containing protein	-
CE10_RS29755	4352825..4354002	+	1178	WP_202945680.1	IS3 family transposase	-
CE10_RS22085	4354083..4354562	+	480	WP_000706978.1	antirestriction protein	-
CE10_RS22090	4354578..4355054	+	477	WP_001186792.1	RadC family protein	-
CE10_RS22095	4355141..4355362	+	222	WP_000692350.1	DUF987 domain-containing protein	-
CE10_RS22100	4355442..4355810	+	369	WP_001285614.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CE10_RS22105	4355899..4356276	+	378	WP_000854823.1	TA system toxin CbtA family protein	Toxin
CE10_RS30295	4356273..4356422	+	150	Protein_4190	hypothetical protein	-
CE10_RS30300	4356498..4356695	+	198	WP_087890155.1	DUF957 domain-containing protein	-
CE10_RS22120	4356780..4357622	+	843	WP_001290170.1	DUF4942 domain-containing protein	-
CE10_RS22125	4357862..4357990	+	129	Protein_4193	virulence RhuM family protein	-
CE10_RS22130	4358419..4359603	+	1185	WP_001172866.1	sugar efflux transporter	-
CE10_RS22135	4359714..4360637	+	924	WP_000535959.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4341954..4369243	27289

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14072.04 Da
• Isoelectric Point: 6.7093

>T27498 WP_000854823.1 NC_017646:4355899-4356276 [Escherichia coli O7:K1 str. CE10]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTHSQLINSIDILRARRATGLMTRDNYRMVNDISQGKYPEAKQ

Nucleotide

• Length: 378 bp

>T27498 NC_017646:4355899-4356276 [Escherichia coli O7:K1 str. CE10]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCACCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAATGGTAAATGACATTAGCCAGGGGAAGTATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13596.35 Da
• Isoelectric Point: 6.0618

>AT27498 WP_001285614.1 NC_017646:4355442-4355810 [Escherichia coli O7:K1 str. CE10]
VSDTLPGTTLPDDNNDRTWWGLPCTVTSCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT27498 NC_017646:4355442-4355810 [Escherichia coli O7:K1 str. CE10]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GTCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA