Detailed information of TA system TA027497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4072252..4072883
• Replicon: chromosome
• Accession: NC_017646
• Organism: Escherichia coli O7:K1 str. CE10

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8T5W4M7
• Locus tag: CE10_RS20735
• Protein ID: WP_001260303.1
• Coordinates: 4072608..4072883 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: CE10_RS20730
• Protein ID: WP_000593555.1
• Coordinates: 4072252..4072611 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CE10_RS20700	4067367..4067840	+	474	WP_001161554.1	PTS sugar transporter subunit IIA	-
CE10_RS20705	4067837..4068118	+	282	WP_000084021.1	PTS sugar transporter subunit IIB	-
CE10_RS20710	4068192..4069550	+	1359	WP_001302220.1	PTS galactitol transporter subunit IIC	-
CE10_RS20715	4069543..4071051	+	1509	WP_012602556.1	carbohydrate kinase	-
CE10_RS20720	4071041..4071310	+	270	WP_000949581.1	HPr family phosphocarrier protein	-
CE10_RS20725	4071342..4072202	+	861	WP_001131789.1	class II aldolase	-
CE10_RS20730	4072252..4072611	-	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CE10_RS20735	4072608..4072883	-	276	WP_001260303.1	type II toxin-antitoxin system HicA family toxin	Toxin
CE10_RS20740	4072956..4074080	-	1125	WP_001314210.1	ABC transporter permease	-
CE10_RS20745	4074080..4076815	-	2736	WP_000149099.1	ribosome-associated ATPase/putative transporter RbbA	-
CE10_RS20750	4076812..4077879	-	1068	WP_000361489.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10167.85 Da
• Isoelectric Point: 10.6128

>T27497 WP_001260303.1 NC_017646:c4072883-4072608 [Escherichia coli O7:K1 str. CE10]
MRTQVQALRKKQKNTLDQIVKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKSAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T27497 NC_017646:c4072883-4072608 [Escherichia coli O7:K1 str. CE10]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAAGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT27497 WP_000593555.1 NC_017646:c4072611-4072252 [Escherichia coli O7:K1 str. CE10]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT27497 NC_017646:c4072611-4072252 [Escherichia coli O7:K1 str. CE10]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6