Detailed information of TA system TA027475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 430707..431544
• Replicon: chromosome
• Accession: NC_017646
• Organism: Escherichia coli O7:K1 str. CE10

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: V0SED1
• Locus tag: CE10_RS02075
• Protein ID: WP_000227787.1
• Coordinates: 430707..431249 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: B1LJI1
• Locus tag: CE10_RS02080
• Protein ID: WP_001353405.1
• Coordinates: 431233..431544 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CE10_RS02055	426247..427158	-	912	WP_000705836.1	2-dehydropantoate 2-reductase	-
CE10_RS02060	427326..427817	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
CE10_RS02065	427945..429309	-	1365	WP_001000978.1	MFS transporter	-
CE10_RS02070	429716..430651	+	936	WP_012311701.1	sel1 repeat family protein	-
CE10_RS02075	430707..431249	-	543	WP_000227787.1	GNAT family N-acetyltransferase	Toxin
CE10_RS02080	431233..431544	-	312	WP_001353405.1	DUF1778 domain-containing protein	Antitoxin
CE10_RS02085	431729..432619	-	891	WP_000971336.1	heme o synthase	-
CE10_RS02090	432631..432960	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
CE10_RS02095	432960..433574	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
CE10_RS02100	433564..435555	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
CE10_RS02105	435577..436524	-	948	WP_001239436.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19946.24 Da
• Isoelectric Point: 8.8951

>T27475 WP_000227787.1 NC_017646:c431249-430707 [Escherichia coli O7:K1 str. CE10]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T27475 NC_017646:c431249-430707 [Escherichia coli O7:K1 str. CE10]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCTGCTAAAGCTCTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTAGGGGTAGCGCG
GAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCGTGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGCCTCGGCTTTGTTCAGCTTTCAGCG
AGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11370.09 Da
• Isoelectric Point: 10.4825

>AT27475 WP_001353405.1 NC_017646:c431544-431233 [Escherichia coli O7:K1 str. CE10]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT27475 NC_017646:c431544-431233 [Escherichia coli O7:K1 str. CE10]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A829GE43

Antitoxin

• AlphaFold DB: A0A829G7G3