Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapB(antitoxin)
Location 9421..9995 Replicon plasmid pUMNK88_Hly
Accession NC_017643
Organism Escherichia coli UMNK88

Toxin (Protein)


Gene name vapC Uniprot ID A0A3A6SGD2
Locus tag UMNK88_RS28090 Protein ID WP_000604346.1
Coordinates 9621..9995 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A3A6S782
Locus tag UMNK88_RS28085 Protein ID WP_001261052.1
Coordinates 9421..9624 (+) Length 68 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UMNK88_RS28060 4600..4857 + 258 WP_000174705.1 hypothetical protein -
UMNK88_RS28065 4912..5548 + 637 Protein_9 DUF4942 domain-containing protein -
UMNK88_RS33605 5593..5763 - 171 WP_071881852.1 helix-turn-helix domain-containing protein -
UMNK88_RS28070 6380..6694 - 315 WP_000741300.1 hypothetical protein -
UMNK88_RS28075 6964..7299 + 336 WP_000045231.1 mobilization protein -
UMNK88_RS28080 7305..9317 + 2013 WP_000635641.1 relaxase/mobilization nuclease domain-containing protein -
UMNK88_RS28085 9421..9624 + 204 WP_001261052.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
UMNK88_RS28090 9621..9995 + 375 WP_000604346.1 PIN domain-containing protein Toxin
UMNK88_RS28095 10177..10734 - 558 WP_000735639.1 Ail/Lom family outer membrane beta-barrel protein -
UMNK88_RS29575 10818..11127 - 310 Protein_17 transposase -
UMNK88_RS28115 11542..12084 - 543 WP_001005723.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyC / hlyA / hlyB / hlyD 1..65549 65549
- flank IS/Tn - - 10975..11127 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-114)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-44)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13911.31 Da        Isoelectric Point: 6.9672

>T27471 WP_000604346.1 NC_017643:9621-9995 [Escherichia coli UMNK88]
MILVDTSVWVDHFKNKNDTLIQLLQSDFVLMHPMILAEIACGTPPAPRQRTLSDLDLLPKSHQATITEVLAFIENEKLFG
LGCGLVDITLLASTRITPGAKIWTLDKRLSRLAKHLNVEYQPVH

Download         Length: 375 bp

>T27471 NC_017643:9621-9995 [Escherichia coli UMNK88]
ATGATATTAGTAGATACGTCTGTGTGGGTTGATCATTTTAAAAATAAAAATGATACATTGATTCAGCTATTGCAGTCAGA
CTTTGTTCTTATGCACCCCATGATCCTGGCTGAAATTGCATGCGGCACACCGCCAGCGCCCAGGCAGCGAACATTGAGTG
ATTTAGACTTGTTACCGAAAAGTCATCAGGCCACGATCACTGAAGTTCTGGCGTTCATTGAAAATGAAAAATTATTCGGA
CTGGGTTGTGGTCTGGTTGATATAACATTATTAGCGTCAACGAGGATTACCCCCGGGGCTAAAATATGGACGCTGGATAA
GCGACTGTCACGACTGGCAAAACACCTGAATGTAGAATATCAGCCTGTACATTGA

Antitoxin


Download         Length: 68 a.a.        Molecular weight: 7366.47 Da        Isoelectric Point: 6.2644

>AT27471 WP_001261052.1 NC_017643:9421-9624 [Escherichia coli UMNK88]
MRTTVTIDDVLYAQALEMADPGMDKSDIFREAVKTFIRVQAAKRLASLGGAAPNMEITPRRRGDVAE

Download         Length: 204 bp

>AT27471 NC_017643:9421-9624 [Escherichia coli UMNK88]
ATGAGAACTACTGTAACGATCGACGATGTTCTGTACGCACAGGCGCTGGAAATGGCTGATCCGGGTATGGATAAATCAGA
TATTTTCCGTGAAGCTGTGAAAACGTTTATTCGTGTGCAGGCTGCGAAACGCCTGGCTTCACTTGGTGGAGCTGCCCCGA
ATATGGAGATAACACCACGCAGGCGTGGAGATGTGGCAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3A6SGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A3A6S782

References