Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 5133230..5133806 Replicon chromosome
Accession NC_017641
Organism Escherichia coli UMNK88

Toxin (Protein)


Gene name shpA Uniprot ID A0A2X2XYK1
Locus tag UMNK88_RS26100 Protein ID WP_001360091.1
Coordinates 5133230..5133517 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag UMNK88_RS26105 Protein ID WP_000063156.1
Coordinates 5133504..5133806 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UMNK88_RS26080 5129483..5130763 - 1281 WP_001037377.1 DUF445 domain-containing protein -
UMNK88_RS26085 5130804..5132036 - 1233 WP_015675357.1 multidrug efflux MFS transporter MdtM -
UMNK88_RS31565 5132182..5132277 - 96 Protein_4947 dienelactone hydrolase family protein -
UMNK88_RS29570 5132334..5133031 + 698 Protein_4948 IS1 family transposase -
UMNK88_RS26100 5133230..5133517 + 288 WP_001360091.1 BrnT family toxin Toxin
UMNK88_RS26105 5133504..5133806 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
UMNK88_RS26110 5133840..5134796 - 957 WP_001297640.1 GTPase -
UMNK88_RS26115 5134807..5135010 - 204 WP_000467859.1 YbdD/YjiX family protein -
UMNK88_RS26120 5135060..5137210 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11325.80 Da        Isoelectric Point: 6.8821

>T27466 WP_001360091.1 NC_017641:5133230-5133517 [Escherichia coli UMNK88]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKTDRKERNRYEHG

Download         Length: 288 bp

>T27466 NC_017641:5133230-5133517 [Escherichia coli UMNK88]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAACAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT27466 WP_000063156.1 NC_017641:5133504-5133806 [Escherichia coli UMNK88]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT27466 NC_017641:5133504-5133806 [Escherichia coli UMNK88]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2X2XYK1

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References