Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4875850..4876347 Replicon chromosome
Accession NC_017641
Organism Escherichia coli UMNK88

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag UMNK88_RS24765 Protein ID WP_001173343.1
Coordinates 4876174..4876347 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag UMNK88_RS24760 Protein ID WP_000398619.1
Coordinates 4875850..4876146 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UMNK88_RS24740 4872581..4873300 - 720 WP_000611288.1 two-component system response regulator DcuR -
UMNK88_RS24745 4873297..4874928 - 1632 WP_001216477.1 sensor histidine kinase -
UMNK88_RS24750 4875109..4875339 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
UMNK88_RS24755 4875351..4875623 + 273 WP_000405651.1 N-acetyltransferase -
UMNK88_RS24760 4875850..4876146 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
UMNK88_RS24765 4876174..4876347 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
UMNK88_RS24770 4876471..4877988 - 1518 WP_001295075.1 lysine--tRNA ligase -
UMNK88_RS24775 4878225..4879682 - 1458 Protein_4697 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T27465 WP_001173343.1 NC_017641:4876174-4876347 [Escherichia coli UMNK88]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T27465 NC_017641:4876174-4876347 [Escherichia coli UMNK88]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT27465 WP_000398619.1 NC_017641:4875850-4876146 [Escherichia coli UMNK88]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT27465 NC_017641:4875850-4876146 [Escherichia coli UMNK88]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References