Detailed information of TA system TA027461

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3580501..3581336
• Replicon: chromosome
• Accession: NC_017641
• Organism: Escherichia coli UMNK88

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: UMNK88_RS18430
• Protein ID: WP_000854821.1
• Coordinates: 3580959..3581336 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: UMNK88_RS18425
• Protein ID: WP_001285593.1
• Coordinates: 3580501..3580869 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UMNK88_RS18405	3577929..3578162	+	234	WP_001117568.1	DUF905 family protein	-
UMNK88_RS18410	3578252..3579070	+	819	WP_001175163.1	DUF945 domain-containing protein	-
UMNK88_RS18415	3579162..3579647	+	486	WP_000206652.1	antirestriction protein	-
UMNK88_RS18420	3579662..3580138	+	477	WP_001186726.1	RadC family protein	-
UMNK88_RS30995	3580201..3580421	+	221	Protein_3512	DUF987 domain-containing protein	-
UMNK88_RS18425	3580501..3580869	+	369	WP_001285593.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
UMNK88_RS18430	3580959..3581336	+	378	WP_000854821.1	type IV toxin-antitoxin system toxin CbtA	Toxin
UMNK88_RS33310	3581333..3581530	+	198	Protein_3515	hypothetical protein	-
UMNK88_RS33315	3581558..3581755	+	198	WP_085949090.1	DUF957 domain-containing protein	-
UMNK88_RS18440	3581840..3582682	+	843	WP_001280493.1	DUF4942 domain-containing protein	-
UMNK88_RS18445	3582963..3583499	-	537	WP_000942783.1	GspM family type II secretion system protein YghD	-
UMNK88_RS18450	3583501..3584679	-	1179	WP_000094962.1	type II secretion system protein GspL	-
UMNK88_RS18455	3584676..3585653	-	978	WP_000633190.1	type II secretion system minor pseudopilin GspK	-
UMNK88_RS18460	3585656..3586255	-	600	WP_024250706.1	type II secretion system minor pseudopilin GspJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T27461 WP_000854821.1 NC_017641:3580959-3581336 [Escherichia coli UMNK88]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T27461 NC_017641:3580959-3581336 [Escherichia coli UMNK88]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13625.44 Da
• Isoelectric Point: 6.3159

>AT27461 WP_001285593.1 NC_017641:3580501-3580869 [Escherichia coli UMNK88]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT27461 NC_017641:3580501-3580869 [Escherichia coli UMNK88]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54