Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3473938..3474592 Replicon chromosome
Accession NC_017641
Organism Escherichia coli UMNK88

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag UMNK88_RS17875 Protein ID WP_000244777.1
Coordinates 3473938..3474345 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag UMNK88_RS17880 Protein ID WP_000354046.1
Coordinates 3474326..3474592 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UMNK88_RS17855 3469895..3471628 - 1734 WP_000813208.1 single-stranded-DNA-specific exonuclease RecJ -
UMNK88_RS17860 3471634..3472344 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
UMNK88_RS17865 3472369..3473265 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
UMNK88_RS17870 3473377..3473898 + 522 WP_001055874.1 flavodoxin FldB -
UMNK88_RS17875 3473938..3474345 - 408 WP_000244777.1 toxin CptA Toxin
UMNK88_RS17880 3474326..3474592 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
UMNK88_RS17885 3474835..3475815 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
UMNK88_RS17890 3476011..3476670 - 660 WP_000250274.1 hemolysin III family protein -
UMNK88_RS17895 3476834..3477145 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
UMNK88_RS17900 3477190..3478623 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
UMNK88_RS17905 3478680..3479423 - 744 WP_000951949.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T27460 WP_000244777.1 NC_017641:c3474345-3473938 [Escherichia coli UMNK88]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T27460 NC_017641:c3474345-3473938 [Escherichia coli UMNK88]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT27460 WP_000354046.1 NC_017641:c3474592-3474326 [Escherichia coli UMNK88]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT27460 NC_017641:c3474592-3474326 [Escherichia coli UMNK88]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References