Detailed information of TA system TA274485

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 17864..19404
• Replicon: plasmid pOB3b_02
• Accession: NZ_CP119871
• Organism: Methylosinus trichosporium OB3b

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: P3C36_RS22460
• Protein ID: WP_283472126.1
• Coordinates: 18520..19404 (+)
• Length: 295 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: P3C36_RS22455
• Protein ID: WP_099832047.1
• Coordinates: 17864..18487 (+)
• Length: 208 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P3C36_RS22435	13626..14489	+	864	WP_099832043.1	patatin-like phospholipase family protein	-
P3C36_RS22440	14521..15234	-	714	WP_099832044.1	SMC-Scp complex subunit ScpB	-
P3C36_RS22445	15244..16332	-	1089	WP_099832045.1	DUF1403 family protein	-
P3C36_RS22450	16404..17534	+	1131	WP_244593424.1	tyrosine-type recombinase/integrase	-
P3C36_RS22455	17864..18487	+	624	WP_099832047.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
P3C36_RS22460	18520..19404	+	885	WP_283472126.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
P3C36_RS22465	19408..20322	+	915	WP_099832049.1	recombinase family protein	-
P3C36_RS22470	20363..21688	-	1326	WP_099832050.1	plasmid replication protein RepC	-
P3C36_RS22475	21877..22860	-	984	WP_099832051.1	plasmid partitioning protein RepB	-
P3C36_RS22480	22857..24059	-	1203	WP_099832115.1	plasmid partitioning protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..209103	209103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (27-250)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (19-61)

Sequences

Toxin

Protein

• Length: 295 a.a.
• Molecular weight: 32677.63 Da
• Isoelectric Point: 5.5164

>T274485 WP_283472126.1 NZ_CP119871:18520-19404 [Methylosinus trichosporium OB3b]
VRDRLTQRARAAGENVQLILTRYAIERLLYRLSASQHRERFVLKGAMLFSLWGPTPYRATGDLDLFGTGNPEIEQLISVF
RAIIQVEVVEDGVAFRSDTLRAEPTRPQDEYAGVRIMFDASIANARLPIQVDIGFGDAITPAPQEIEYPSLIGMPAPKLK
AYPPETVVAEKLEALVSLGVANSRMKDFYDLWAISRTFAFDGLVLTKAIRATFERRGTMLPTEIPFGLSEAFAADPVKQT
QWRAFLRRTEIAMAPEPLTQIIPSIASFLMPVLQSAADERTTPGKWPVGGPWGD

Nucleotide

• Length: 885 bp

Antitoxin

Protein

• Length: 208 a.a.
• Molecular weight: 22820.54 Da
• Isoelectric Point: 10.5683

>AT274485 WP_099832047.1 NZ_CP119871:17864-18487 [Methylosinus trichosporium OB3b]
MQQSPTIQAKSLKELALAVARDRGIARSRDFEAAGVPRIYLQRLRDEGLLTQPGRGLYALANSQISAHHSLAEAAKSVPS
GVIGLLSALQFHELTTQLPSQVWMLLPSKAWAPRRSPVTLKILRASGEALKAGVEQQLVDRVVVPITSPAKTVADCFKYR
GKIGLDVAIEALRDCLTKKRATRDEIWRYAAIDRVQNVMRPYLEALS

Nucleotide

• Length: 624 bp