Detailed information of TA system TA027448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1936689..1937215
• Replicon: chromosome
• Accession: NC_017641
• Organism: Escherichia coli UMNK88

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: UMNK88_RS09945
• Protein ID: WP_000323025.1
• Coordinates: 1936689..1936976 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: UMNK88_RS09950
• Protein ID: WP_000534858.1
• Coordinates: 1936976..1937215 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UMNK88_RS09910	1931713..1931928	-	216	WP_000839590.1	phage lysis protein EssD	-
UMNK88_RS09915	1932682..1932897	-	216	WP_000066484.1	cold shock-like protein CspB	-
UMNK88_RS09920	1933198..1933410	+	213	WP_000087756.1	cold shock-like protein CspF	-
UMNK88_RS33840	1933465..1933554	+	90	WP_120795389.1	hypothetical protein	-
UMNK88_RS09925	1933832..1934584	-	753	WP_001047136.1	antitermination protein	-
UMNK88_RS09930	1934598..1935647	-	1050	WP_001424248.1	DUF968 domain-containing protein	-
UMNK88_RS30395	1935649..1935927	-	279	WP_012304870.1	hypothetical protein	-
UMNK88_RS09935	1935994..1936245	-	252	WP_000980994.1	hypothetical protein	-
UMNK88_RS09940	1936462..1936617	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
UMNK88_RS09945	1936689..1936976	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
UMNK88_RS09950	1936976..1937215	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
UMNK88_RS30400	1937240..1937545	+	306	WP_001326990.1	hypothetical protein	-
UMNK88_RS09955	1937748..1938080	+	333	WP_001301033.1	protein FlxA	-
UMNK88_RS09960	1938517..1939830	-	1314	WP_000589009.1	ISNCY family transposase	-
UMNK88_RS34365	1940599..1940886	-	288	Protein_1911	hypothetical protein	-
UMNK88_RS09975	1941497..1941853	-	357	WP_001310834.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1899408..1967807	68399

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T27448 WP_000323025.1 NC_017641:c1936976-1936689 [Escherichia coli UMNK88]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T27448 NC_017641:c1936976-1936689 [Escherichia coli UMNK88]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT27448 WP_000534858.1 NC_017641:c1937215-1936976 [Escherichia coli UMNK88]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT27448 NC_017641:c1937215-1936976 [Escherichia coli UMNK88]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8