Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 209395..211018 Replicon plasmid pE15-1
Accession NZ_CP119747
Organism Escherichia coli strain E15

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag P1J19_RS25170 Protein ID WP_000088046.1
Coordinates 209693..211018 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag P1J19_RS25165 Protein ID WP_024136327.1
Coordinates 209395..209688 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1J19_RS25135 (P1J19_25135) 205267..206508 + 1242 WP_001053338.1 VWA domain-containing protein -
P1J19_RS25140 (P1J19_25140) 206599..206730 + 132 WP_000374059.1 hypothetical protein -
P1J19_RS25145 (P1J19_25145) 207071..207352 - 282 WP_000077926.1 hypothetical protein -
P1J19_RS25150 (P1J19_25150) 207402..207593 - 192 WP_000398480.1 hypothetical protein -
P1J19_RS25155 (P1J19_25155) 207685..208026 - 342 WP_001151575.1 hypothetical protein -
P1J19_RS25160 (P1J19_25160) 208399..208791 + 393 WP_000341066.1 hypothetical protein -
P1J19_RS25165 (P1J19_25165) 209395..209688 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
P1J19_RS25170 (P1J19_25170) 209693..211018 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
P1J19_RS25175 (P1J19_25175) 211079..211285 + 207 WP_000134171.1 hypothetical protein -
P1J19_RS25180 (P1J19_25180) 211387..211797 + 411 WP_000985911.1 hypothetical protein -
P1J19_RS25185 (P1J19_25185) 211810..212055 + 246 Protein_217 HNH endonuclease -
P1J19_RS25190 (P1J19_25190) 212108..212812 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
P1J19_RS25195 (P1J19_25195) 212859..213434 - 576 WP_072740834.1 recombinase family protein -
P1J19_RS25200 (P1J19_25200) 213560..213910 - 351 WP_000454193.1 DUF3330 domain-containing protein -
P1J19_RS25205 (P1J19_25205) 214177..214881 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
P1J19_RS25210 (P1J19_25210) 214933..215163 + 231 Protein_222 aminoglycoside phosphotransferase family protein -
P1J19_RS25215 (P1J19_25215) 215249..215362 - 114 Protein_223 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(3'')-Ia / cmlA1 / aadA2 / blaTEM-1B / tet(A) / qnrS1 / ARR-3 / blaOXA-10 / dfrA14 / mcr-1.1 / aph(3')-Ia / aph(4)-Ia / aac(3)-IVa / sul3 - 1..226260 226260


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T274409 WP_000088046.1 NZ_CP119747:209693-211018 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT274409 WP_024136327.1 NZ_CP119747:209395-209688 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References