Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3647833..3648670 Replicon chromosome
Accession NZ_CP119746
Organism Escherichia coli strain E15

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag P1J19_RS17785 Protein ID WP_000227784.1
Coordinates 3648128..3648670 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag P1J19_RS17780 Protein ID WP_001297137.1
Coordinates 3647833..3648144 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1J19_RS17755 (3642853) 3642853..3643800 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
P1J19_RS17760 (3643822) 3643822..3645813 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
P1J19_RS17765 (3645803) 3645803..3646417 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
P1J19_RS17770 (3646417) 3646417..3646746 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
P1J19_RS17775 (3646758) 3646758..3647648 + 891 WP_000971336.1 heme o synthase -
P1J19_RS17780 (3647833) 3647833..3648144 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
P1J19_RS17785 (3648128) 3648128..3648670 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
P1J19_RS17795 (3649613) 3649613..3650437 - 825 Protein_3481 tetratricopeptide repeat protein -
P1J19_RS17800 (3650845) 3650845..3652209 + 1365 WP_001000974.1 MFS transporter -
P1J19_RS17805 (3652443) 3652443..3653423 + 981 WP_000399648.1 IS110-like element IS621 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T274404 WP_000227784.1 NZ_CP119746:3648128-3648670 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT274404 WP_001297137.1 NZ_CP119746:3647833-3648144 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References