Detailed information of TA system TA027439

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1275713..1276547
• Replicon: chromosome
• Accession: NC_017641
• Organism: Escherichia coli UMNK88

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: W0S379
• Locus tag: UMNK88_RS06355
• Protein ID: WP_000854808.1
• Coordinates: 1276170..1276547 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: W0S4A5
• Locus tag: UMNK88_RS06350
• Protein ID: WP_001285114.1
• Coordinates: 1275713..1276081 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UMNK88_RS06310	1271187..1271726	+	540	WP_001104020.1	DUF4339 domain-containing protein	-
UMNK88_RS06315	1271996..1272409	+	414	WP_000789535.1	hypothetical protein	-
UMNK88_RS33050	1272507..1272730	+	224	Protein_1201	DUF905 family protein	-
UMNK88_RS06325	1272820..1273638	+	819	WP_001234743.1	DUF945 domain-containing protein	-
UMNK88_RS06330	1273730..1274215	+	486	WP_000214416.1	antirestriction protein	-
UMNK88_RS06335	1274231..1274707	+	477	WP_001186747.1	RadC family protein	-
UMNK88_RS06340	1274776..1274997	+	222	WP_000692345.1	DUF987 domain-containing protein	-
UMNK88_RS06345	1275016..1275663	+	648	WP_000094919.1	hypothetical protein	-
UMNK88_RS06350	1275713..1276081	+	369	WP_001285114.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
UMNK88_RS06355	1276170..1276547	+	378	WP_000854808.1	type IV toxin-antitoxin system toxin CbtA	Toxin
UMNK88_RS06360	1276544..1277032	+	489	WP_000761657.1	hypothetical protein	-
UMNK88_RS06365	1277044..1277241	+	198	WP_000839248.1	DUF957 domain-containing protein	-
UMNK88_RS06370	1277326..1278168	+	843	WP_001280436.1	DUF4942 domain-containing protein	-
UMNK88_RS06380	1278639..1279577	+	939	WP_000351315.1	glyoxylate/hydroxypyruvate reductase GhrA	-
UMNK88_RS06385	1279632..1280369	+	738	WP_000283664.1	zinc-binding phosphatase	-
UMNK88_RS06390	1280393..1280947	+	555	WP_001001902.1	molecular chaperone YcdY	-
UMNK88_RS06395	1281049..1281540	+	492	WP_001300785.1	DUF1097 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13909.93 Da
• Isoelectric Point: 7.9085

>T27439 WP_000854808.1 NC_017641:1276170-1276547 [Escherichia coli UMNK88]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T27439 NC_017641:1276170-1276547 [Escherichia coli UMNK88]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13767.60 Da
• Isoelectric Point: 6.9460

>AT27439 WP_001285114.1 NC_017641:1275713-1276081 [Escherichia coli UMNK88]
VSDSLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQALPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCKADTLSSCGYVYLAVYPTPKMKN

Nucleotide

• Length: 369 bp

>AT27439 NC_017641:1275713-1276081 [Escherichia coli UMNK88]
GTGTCAGACTCACTCCATGAAACAAATTATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCCTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCAAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: W0S379

Antitoxin

• AlphaFold DB: W0S4A5