Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 29913..30514 Replicon plasmid pE94-3
Accession NZ_CP119743
Organism Escherichia coli strain E94

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag P1J23_RS27640 Protein ID WP_001216045.1
Coordinates 29913..30293 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag P1J23_RS27645 Protein ID WP_001190712.1
Coordinates 30293..30514 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1J23_RS27615 (P1J23_27615) 25353..26837 - 1485 WP_000124150.1 terminase -
P1J23_RS27620 (P1J23_27620) 26837..28030 - 1194 WP_000219607.1 hypothetical protein -
P1J23_RS27625 (P1J23_27625) 28117..28569 - 453 WP_016236875.1 hypothetical protein -
P1J23_RS27630 (P1J23_27630) 28658..29701 - 1044 WP_000648821.1 DUF968 domain-containing protein -
P1J23_RS27635 (P1J23_27635) 29729..29908 - 180 WP_000113018.1 hypothetical protein -
P1J23_RS27640 (P1J23_27640) 29913..30293 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
P1J23_RS27645 (P1J23_27645) 30293..30514 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
P1J23_RS27650 (P1J23_27650) 30697..32253 + 1557 WP_147707485.1 type I restriction-modification system subunit M -
P1J23_RS27655 (P1J23_27655) 32250..33455 + 1206 WP_001293319.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..100470 100470


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T274386 WP_001216045.1 NZ_CP119743:c30293-29913 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT274386 WP_001190712.1 NZ_CP119743:c30514-30293 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References