Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 172353..173976 Replicon plasmid pE94-1
Accession NZ_CP119741
Organism Escherichia coli strain E94

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag P1J23_RS26505 Protein ID WP_000088046.1
Coordinates 172651..173976 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag P1J23_RS26500 Protein ID WP_024136327.1
Coordinates 172353..172646 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1J23_RS26470 (P1J23_26470) 168225..169466 + 1242 WP_001053338.1 VWA domain-containing protein -
P1J23_RS26475 (P1J23_26475) 169557..169688 + 132 WP_000374059.1 hypothetical protein -
P1J23_RS26480 (P1J23_26480) 170029..170310 - 282 WP_000077926.1 hypothetical protein -
P1J23_RS26485 (P1J23_26485) 170360..170551 - 192 WP_000398480.1 hypothetical protein -
P1J23_RS26490 (P1J23_26490) 170643..170984 - 342 WP_001151575.1 hypothetical protein -
P1J23_RS26495 (P1J23_26495) 171357..171749 + 393 WP_000341066.1 hypothetical protein -
P1J23_RS26500 (P1J23_26500) 172353..172646 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
P1J23_RS26505 (P1J23_26505) 172651..173976 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
P1J23_RS26510 (P1J23_26510) 174037..174243 + 207 WP_000134171.1 hypothetical protein -
P1J23_RS26515 (P1J23_26515) 174345..174755 + 411 WP_000985911.1 hypothetical protein -
P1J23_RS26520 (P1J23_26520) 174768..175583 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
P1J23_RS26525 (P1J23_26525) 175837..176262 + 426 WP_001043843.1 hypothetical protein -
P1J23_RS26530 (P1J23_26530) 176716..176952 + 237 Protein_182 type I restriction-modification system subunit M N-terminal domain-containing protein -
P1J23_RS26535 (P1J23_26535) 177007..177306 + 300 WP_001572440.1 hypothetical protein -
P1J23_RS26540 (P1J23_26540) 177296..177616 + 321 WP_123872853.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA2 / cmlA1 / ant(3'')-Ia / sul3 / sul1 / oqxA / oqxB - 1..231243 231243


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T274382 WP_000088046.1 NZ_CP119741:172651-173976 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT274382 WP_024136327.1 NZ_CP119741:172353-172646 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References