Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 63295..63817 Replicon plasmid pE92-2
Accession NZ_CP119736
Organism Escherichia coli strain E92

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag P1J22_RS27230 Protein ID WP_000220560.1
Coordinates 63536..63817 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag P1J22_RS27225 Protein ID WP_000121743.1
Coordinates 63295..63546 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1J22_RS27205 (59190) 59190..59555 + 366 WP_000091308.1 hypothetical protein -
P1J22_RS27210 (59555) 59555..60744 + 1190 Protein_70 IS91 family transposase -
P1J22_RS27215 (60934) 60934..61185 - 252 Protein_71 hypothetical protein -
P1J22_RS27220 (61225) 61225..62061 - 837 WP_001050931.1 RepB family plasmid replication initiator protein -
P1J22_RS27225 (63295) 63295..63546 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
P1J22_RS27230 (63536) 63536..63817 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
P1J22_RS27235 (63963) 63963..64286 + 324 WP_001181907.1 hypothetical protein -
P1J22_RS27240 (64331) 64331..64576 + 246 WP_000356545.1 hypothetical protein -
P1J22_RS27245 (64566) 64566..64781 + 216 WP_001180116.1 hypothetical protein -
P1J22_RS27250 (65170) 65170..66357 - 1188 WP_000937591.1 IS91 family transposase -
P1J22_RS27255 (66357) 66357..66722 - 366 WP_000091308.1 hypothetical protein -
P1J22_RS27260 (66999) 66999..68186 - 1188 WP_276171324.1 IS91-like element IS91 family transposase -
P1J22_RS27265 (68186) 68186..68551 - 366 WP_000124098.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - estIa 1..101028 101028
- inside IScluster/Tn - estIa 57726..84826 27100


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T274354 WP_000220560.1 NZ_CP119736:63536-63817 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT274354 WP_000121743.1 NZ_CP119736:63295-63546 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References