Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 111949..113572 Replicon plasmid pE92-1
Accession NZ_CP119735
Organism Escherichia coli strain E92

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag P1J22_RS26155 Protein ID WP_000088046.1
Coordinates 112247..113572 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag P1J22_RS26150 Protein ID WP_024136327.1
Coordinates 111949..112242 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1J22_RS26120 (P1J22_26120) 107821..109062 + 1242 WP_001053338.1 VWA domain-containing protein -
P1J22_RS26125 (P1J22_26125) 109153..109284 + 132 WP_000374059.1 hypothetical protein -
P1J22_RS26130 (P1J22_26130) 109625..109906 - 282 WP_000077926.1 hypothetical protein -
P1J22_RS26135 (P1J22_26135) 109956..110147 - 192 WP_000398480.1 hypothetical protein -
P1J22_RS26140 (P1J22_26140) 110239..110580 - 342 WP_001151575.1 hypothetical protein -
P1J22_RS26145 (P1J22_26145) 110953..111345 + 393 WP_000341066.1 hypothetical protein -
P1J22_RS26150 (P1J22_26150) 111949..112242 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
P1J22_RS26155 (P1J22_26155) 112247..113572 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
P1J22_RS26160 (P1J22_26160) 113633..113839 + 207 WP_000134171.1 hypothetical protein -
P1J22_RS26165 (P1J22_26165) 113941..114351 + 411 WP_000985911.1 hypothetical protein -
P1J22_RS26170 (P1J22_26170) 114364..115179 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
P1J22_RS26175 (P1J22_26175) 115433..115858 + 426 WP_001043843.1 hypothetical protein -
P1J22_RS26180 (P1J22_26180) 116312..116548 + 237 Protein_112 type I restriction-modification system subunit M N-terminal domain-containing protein -
P1J22_RS26185 (P1J22_26185) 116603..116902 + 300 WP_001572440.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA2 / cmlA1 / ant(3'')-Ia / sul3 / sul1 / oqxA / oqxB - 1..231254 231254


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T274352 WP_000088046.1 NZ_CP119735:112247-113572 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT274352 WP_024136327.1 NZ_CP119735:111949-112242 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References