Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3128716..3129370 Replicon chromosome
Accession NZ_CP119734
Organism Escherichia coli strain E92

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag P1J22_RS15775 Protein ID WP_000244777.1
Coordinates 3128716..3129123 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag P1J22_RS15780 Protein ID WP_000354046.1
Coordinates 3129104..3129370 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1J22_RS15755 (3124673) 3124673..3126406 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
P1J22_RS15760 (3126412) 3126412..3127122 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
P1J22_RS15765 (3127147) 3127147..3128043 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
P1J22_RS15770 (3128155) 3128155..3128676 + 522 WP_001055874.1 flavodoxin FldB -
P1J22_RS15775 (3128716) 3128716..3129123 - 408 WP_000244777.1 protein YgfX Toxin
P1J22_RS15780 (3129104) 3129104..3129370 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
P1J22_RS15785 (3129613) 3129613..3130593 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
P1J22_RS15790 (3130670) 3130670..3131329 - 660 WP_000250274.1 hemolysin III family protein -
P1J22_RS15795 (3131493) 3131493..3131804 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
P1J22_RS15800 (3131849) 3131849..3133282 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
P1J22_RS15805 (3133339) 3133339..3133470 - 132 Protein_3100 SDR family oxidoreductase -
P1J22_RS15810 (3133525) 3133525..3133703 - 179 Protein_3101 helix-turn-helix domain-containing protein -
P1J22_RS15815 (3133711) 3133711..3134082 - 372 WP_244599612.1 tail fiber assembly protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - nueA / msbA 3091965..3275191 183226


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T274346 WP_000244777.1 NZ_CP119734:c3129123-3128716 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT274346 WP_000354046.1 NZ_CP119734:c3129370-3129104 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References