Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 52972..53507 Replicon plasmid pE32-3
Accession NZ_CP119733
Organism Escherichia coli strain E32

Toxin (Protein)


Gene name relE Uniprot ID A0A0D8WEY8
Locus tag P1J21_RS26390 Protein ID WP_032353555.1
Coordinates 52972..53259 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0D8WHE0
Locus tag P1J21_RS26395 Protein ID WP_045145550.1
Coordinates 53256..53507 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1J21_RS26370 (P1J21_26370) 48153..49340 + 1188 WP_045145541.1 IS91 family transposase -
P1J21_RS26375 (P1J21_26375) 50194..50787 + 594 WP_032353553.1 ProQ/FINO family protein -
P1J21_RS26380 (P1J21_26380) 51067..51654 - 588 WP_064550130.1 colanic acid biosynthesis acetyltransferase -
P1J21_RS26385 (P1J21_26385) 52675..52962 - 288 WP_045145547.1 hypothetical protein -
P1J21_RS26390 (P1J21_26390) 52972..53259 - 288 WP_032353555.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
P1J21_RS26395 (P1J21_26395) 53256..53507 - 252 WP_045145550.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
P1J21_RS26400 (P1J21_26400) 53594..53761 - 168 WP_065898644.1 hypothetical protein -
P1J21_RS26405 (P1J21_26405) 54456..55328 - 873 WP_045145554.1 incFII family plasmid replication initiator RepA -
P1J21_RS26410 (P1J21_26410) 56164..56670 + 507 WP_024187405.1 transcription termination/antitermination NusG family protein -
P1J21_RS26415 (P1J21_26415) 56811..57476 + 666 WP_065203167.1 traC -
P1J21_RS26420 (P1J21_26420) 57743..58363 + 621 WP_032353888.1 DUF1449 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..129739 129739
- inside IScluster/Tn - - 5688..49340 43652


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11114.16 Da        Isoelectric Point: 10.6261

>T274329 WP_032353555.1 NZ_CP119733:c53259-52972 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKAIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR

Download         Length: 288 bp

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9219.48 Da        Isoelectric Point: 4.1789

>AT274329 WP_045145550.1 NZ_CP119733:c53507-53256 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHYVDLD
SYL

Download         Length: 252 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8WEY8

Antitoxin

Source ID Structure
AlphaFold DB A0A0D8WHE0

References