Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 176001..177624 Replicon plasmid pE32-1
Accession NZ_CP119731
Organism Escherichia coli strain E32

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag P1J21_RS25090 Protein ID WP_000088046.1
Coordinates 176001..177326 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag P1J21_RS25095 Protein ID WP_024136327.1
Coordinates 177331..177624 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1J21_RS25045 (P1J21_25045) 171657..171770 + 114 Protein_173 hypothetical protein -
P1J21_RS25050 (P1J21_25050) 171856..172086 - 231 Protein_174 aminoglycoside phosphotransferase family protein -
P1J21_RS25055 (P1J21_25055) 172138..172842 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
P1J21_RS25060 (P1J21_25060) 173109..173459 + 351 WP_000454193.1 DUF3330 domain-containing protein -
P1J21_RS25065 (P1J21_25065) 173585..174160 + 576 WP_072740834.1 recombinase family protein -
P1J21_RS25070 (P1J21_25070) 174207..174911 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
P1J21_RS25075 (P1J21_25075) 174964..175209 - 246 Protein_179 HNH endonuclease -
P1J21_RS25080 (P1J21_25080) 175222..175632 - 411 WP_000985911.1 hypothetical protein -
P1J21_RS25085 (P1J21_25085) 175734..175940 - 207 WP_000134171.1 hypothetical protein -
P1J21_RS25090 (P1J21_25090) 176001..177326 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
P1J21_RS25095 (P1J21_25095) 177331..177624 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
P1J21_RS25100 (P1J21_25100) 178228..178620 - 393 WP_000341066.1 hypothetical protein -
P1J21_RS25105 (P1J21_25105) 178993..179334 + 342 WP_001151575.1 hypothetical protein -
P1J21_RS25110 (P1J21_25110) 179426..179617 + 192 WP_000398480.1 hypothetical protein -
P1J21_RS25115 (P1J21_25115) 179667..179948 + 282 WP_000077926.1 hypothetical protein -
P1J21_RS25120 (P1J21_25120) 180289..180420 - 132 WP_000374059.1 hypothetical protein -
P1J21_RS25125 (P1J21_25125) 180511..181752 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA14 / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 / qnrS1 / tet(A) / blaTEM-1B / aadA2 / sul3 / aac(3)-IVa / aph(4)-Ia / aph(3')-Ia / mcr-1.1 - 1..226260 226260


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T274319 WP_000088046.1 NZ_CP119731:c177326-176001 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT274319 WP_024136327.1 NZ_CP119731:c177624-177331 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References