Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3659080..3659917 Replicon chromosome
Accession NZ_CP119730
Organism Escherichia coli strain E32

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag P1J21_RS17865 Protein ID WP_000227784.1
Coordinates 3659375..3659917 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag P1J21_RS17860 Protein ID WP_001297137.1
Coordinates 3659080..3659391 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1J21_RS17835 (3654100) 3654100..3655047 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
P1J21_RS17840 (3655069) 3655069..3657060 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
P1J21_RS17845 (3657050) 3657050..3657664 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
P1J21_RS17850 (3657664) 3657664..3657993 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
P1J21_RS17855 (3658005) 3658005..3658895 + 891 WP_000971336.1 heme o synthase -
P1J21_RS17860 (3659080) 3659080..3659391 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
P1J21_RS17865 (3659375) 3659375..3659917 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
P1J21_RS17875 (3660860) 3660860..3661684 - 825 Protein_3497 tetratricopeptide repeat protein -
P1J21_RS17880 (3662092) 3662092..3663456 + 1365 WP_001000974.1 MFS transporter -
P1J21_RS17885 (3663690) 3663690..3664670 + 981 WP_000399648.1 IS110-like element IS621 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T274314 WP_000227784.1 NZ_CP119730:3659375-3659917 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT274314 WP_001297137.1 NZ_CP119730:3659080-3659391 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References