Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 101458..101983 Replicon plasmid pE16-3
Accession NZ_CP119729
Organism Escherichia coli strain E16

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag P1J20_RS26625 Protein ID WP_001159871.1
Coordinates 101678..101983 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag P1J20_RS26620 Protein ID WP_000813630.1
Coordinates 101458..101676 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1J20_RS26590 (P1J20_26590) 96819..97049 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
P1J20_RS26595 (P1J20_26595) 97046..97462 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
P1J20_RS26600 (P1J20_26600) 98024..99237 + 1214 WP_219795283.1 IS3 family transposase -
P1J20_RS26605 (P1J20_26605) 99628..100080 + 453 WP_032353631.1 acyltransferase -
P1J20_RS26610 (P1J20_26610) 100112..100297 - 186 WP_032353630.1 hypothetical protein -
P1J20_RS26615 (P1J20_26615) 100345..100476 - 132 Protein_106 transposase -
P1J20_RS26620 (P1J20_26620) 101458..101676 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
P1J20_RS26625 (P1J20_26625) 101678..101983 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
P1J20_RS26630 (P1J20_26630) 101984..102793 + 810 WP_045145707.1 site-specific integrase -
P1J20_RS26635 (P1J20_26635) 102966..103319 + 354 WP_000864812.1 colicin M immunity protein -
P1J20_RS26640 (P1J20_26640) 103369..104184 - 816 WP_032494159.1 lipid II-degrading bacteriocin colicin M -
P1J20_RS26645 (P1J20_26645) 104426..104953 + 528 WP_032353548.1 colicin B immunity protein -
P1J20_RS26650 (P1J20_26650) 104971..105633 - 663 Protein_113 colicin-like pore-forming protein -
P1J20_RS26655 (P1J20_26655) 105691..106388 + 698 WP_249940384.1 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..129738 129738


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T274299 WP_001159871.1 NZ_CP119729:101678-101983 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT274299 WP_000813630.1 NZ_CP119729:101458-101676 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References