Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 13657..15280 Replicon plasmid pE16-1
Accession NZ_CP119727
Organism Escherichia coli strain E16

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag P1J20_RS24260 Protein ID WP_000088046.1
Coordinates 13955..15280 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag P1J20_RS24255 Protein ID WP_024136327.1
Coordinates 13657..13950 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1J20_RS24225 (P1J20_24225) 9529..10770 + 1242 WP_001053338.1 VWA domain-containing protein -
P1J20_RS24230 (P1J20_24230) 10861..10992 + 132 WP_000374059.1 hypothetical protein -
P1J20_RS24235 (P1J20_24235) 11333..11614 - 282 WP_000077926.1 hypothetical protein -
P1J20_RS24240 (P1J20_24240) 11664..11855 - 192 WP_000398480.1 hypothetical protein -
P1J20_RS24245 (P1J20_24245) 11947..12288 - 342 WP_001151575.1 hypothetical protein -
P1J20_RS24250 (P1J20_24250) 12661..13053 + 393 WP_000341066.1 hypothetical protein -
P1J20_RS24255 (P1J20_24255) 13657..13950 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
P1J20_RS24260 (P1J20_24260) 13955..15280 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
P1J20_RS24265 (P1J20_24265) 15341..15547 + 207 WP_000134171.1 hypothetical protein -
P1J20_RS24270 (P1J20_24270) 15649..16059 + 411 WP_000985911.1 hypothetical protein -
P1J20_RS24275 (P1J20_24275) 16072..16317 + 246 Protein_21 HNH endonuclease -
P1J20_RS24280 (P1J20_24280) 16370..17074 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
P1J20_RS24285 (P1J20_24285) 17121..17696 - 576 WP_072740834.1 recombinase family protein -
P1J20_RS24290 (P1J20_24290) 17822..18172 - 351 WP_000454193.1 DUF3330 domain-containing protein -
P1J20_RS24295 (P1J20_24295) 18439..19143 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
P1J20_RS24300 (P1J20_24300) 19195..19425 + 231 Protein_26 aminoglycoside phosphotransferase family protein -
P1J20_RS24305 (P1J20_24305) 19511..19624 - 114 Protein_27 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / aph(4)-Ia / aac(3)-IVa / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / blaTEM-1B / tet(A) / qnrS1 / ARR-3 / blaOXA-10 / dfrA14 / mcr-1.1 - 1..226260 226260


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T274288 WP_000088046.1 NZ_CP119727:13955-15280 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT274288 WP_024136327.1 NZ_CP119727:13657-13950 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References