Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3447314..3447951 Replicon chromosome
Accession NZ_CP119675
Organism Bacillus velezensis strain 160

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag PX690_RS17305 Protein ID WP_003156187.1
Coordinates 3447601..3447951 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag PX690_RS17300 Protein ID WP_003156188.1
Coordinates 3447314..3447595 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PX690_RS17280 (3443679) 3443679..3444278 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
PX690_RS17285 (3444371) 3444371..3444736 + 366 WP_003156192.1 holo-ACP synthase -
PX690_RS17290 (3444901) 3444901..3445908 + 1008 WP_276123255.1 outer membrane lipoprotein carrier protein LolA -
PX690_RS17295 (3446025) 3446025..3447194 + 1170 WP_003156189.1 alanine racemase -
PX690_RS17300 (3447314) 3447314..3447595 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
PX690_RS17305 (3447601) 3447601..3447951 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PX690_RS17310 (3448069) 3448069..3448890 + 822 WP_003156182.1 STAS domain-containing protein -
PX690_RS17315 (3448895) 3448895..3449260 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
PX690_RS17320 (3449263) 3449263..3449664 + 402 WP_003156178.1 anti-sigma regulatory factor -
PX690_RS17325 (3449676) 3449676..3450683 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
PX690_RS17330 (3450747) 3450747..3451076 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
PX690_RS17335 (3451073) 3451073..3451555 + 483 WP_003156173.1 anti-sigma B factor RsbW -
PX690_RS17340 (3451521) 3451521..3452309 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
PX690_RS17345 (3452309) 3452309..3452911 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T274265 WP_003156187.1 NZ_CP119675:3447601-3447951 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT274265 WP_003156188.1 NZ_CP119675:3447314-3447595 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References