274254

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1253451..1254368 Replicon chromosome
Accession NZ_CP119596
Organism Bacillus amyloliquefaciens strain MG-2

Toxin (Protein)


Gene name spoIISA Uniprot ID I2HQ15
Locus tag P1K87_RS06565 Protein ID WP_007407256.1
Coordinates 1253622..1254368 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag P1K87_RS06560 Protein ID WP_003154807.1
Coordinates 1253451..1253621 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1K87_RS06520 (P1K87_06520) 1248684..1250306 + 1623 WP_032874601.1 pyocin knob domain-containing protein -
P1K87_RS06525 (P1K87_06525) 1250319..1250690 + 372 WP_032874603.1 XkdW family protein -
P1K87_RS06530 (P1K87_06530) 1250695..1250892 + 198 WP_032874605.1 XkdX family protein -
P1K87_RS06535 (P1K87_06535) 1250949..1251710 + 762 WP_032874607.1 hypothetical protein -
P1K87_RS06540 (P1K87_06540) 1251762..1252025 + 264 WP_003154815.1 hemolysin XhlA family protein -
P1K87_RS06545 (P1K87_06545) 1252039..1252302 + 264 WP_003154813.1 phage holin -
P1K87_RS06550 (P1K87_06550) 1252316..1253194 + 879 WP_032874609.1 N-acetylmuramoyl-L-alanine amidase -
P1K87_RS06555 (P1K87_06555) 1253229..1253354 - 126 WP_003154809.1 hypothetical protein -
P1K87_RS06560 (P1K87_06560) 1253451..1253621 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
P1K87_RS06565 (P1K87_06565) 1253622..1254368 - 747 WP_007407256.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
P1K87_RS06570 (P1K87_06570) 1254473..1255471 - 999 WP_032874611.1 inorganic phosphate transporter -
P1K87_RS06575 (P1K87_06575) 1255484..1256101 - 618 WP_032874614.1 DUF47 domain-containing protein -
P1K87_RS06580 (P1K87_06580) 1256387..1257703 - 1317 WP_032874616.1 amino acid permease -
P1K87_RS06585 (P1K87_06585) 1258025..1258975 + 951 WP_032874618.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29077.57 Da        Isoelectric Point: 4.7755

>T274254 WP_007407256.1 NZ_CP119596:c1254368-1253622 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT274254 WP_003154807.1 NZ_CP119596:c1253621-1253451 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I2HQ15

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References