Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 502211..502848 Replicon chromosome
Accession NZ_CP119596
Organism Bacillus amyloliquefaciens strain MG-2

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag P1K87_RS02585 Protein ID WP_003156187.1
Coordinates 502498..502848 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag P1K87_RS02580 Protein ID WP_003156188.1
Coordinates 502211..502492 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1K87_RS02560 (P1K87_02560) 498576..499175 - 600 WP_032872997.1 rhomboid family intramembrane serine protease -
P1K87_RS02565 (P1K87_02565) 499268..499633 + 366 WP_007609580.1 holo-ACP synthase -
P1K87_RS02570 (P1K87_02570) 499798..500805 + 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
P1K87_RS02575 (P1K87_02575) 500922..502091 + 1170 WP_032873001.1 alanine racemase -
P1K87_RS02580 (P1K87_02580) 502211..502492 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
P1K87_RS02585 (P1K87_02585) 502498..502848 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
P1K87_RS02590 (P1K87_02590) 502966..503787 + 822 WP_003156182.1 STAS domain-containing protein -
P1K87_RS02595 (P1K87_02595) 503792..504157 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
P1K87_RS02600 (P1K87_02600) 504160..504561 + 402 WP_003156178.1 anti-sigma regulatory factor -
P1K87_RS02605 (P1K87_02605) 504573..505580 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
P1K87_RS02610 (P1K87_02610) 505644..505973 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
P1K87_RS02615 (P1K87_02615) 505970..506452 + 483 WP_007609591.1 anti-sigma B factor RsbW -
P1K87_RS02620 (P1K87_02620) 506418..507206 + 789 WP_032873003.1 RNA polymerase sigma factor SigB -
P1K87_RS02625 (P1K87_02625) 507206..507808 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T274253 WP_003156187.1 NZ_CP119596:502498-502848 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT274253 WP_003156188.1 NZ_CP119596:502211-502492 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References