27425

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4428217..4428812 Replicon chromosome
Accession NC_017638
Organism Escherichia coli DH1

Toxin (Protein)


Gene name chpB Uniprot ID U9XNP6
Locus tag ECDH1ME8569_RS21795 Protein ID WP_000239577.1
Coordinates 4428462..4428812 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag ECDH1ME8569_RS21790 Protein ID WP_001223208.1
Coordinates 4428217..4428468 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH1ME8569_RS21780 (ECDH1ME8569_4078) 4423882..4427661 + 3780 WP_000060911.1 autotransporter assembly complex protein TamB -
ECDH1ME8569_RS21785 (ECDH1ME8569_4079) 4427664..4428005 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
ECDH1ME8569_RS21790 (ECDH1ME8569_4081) 4428217..4428468 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
ECDH1ME8569_RS21795 (ECDH1ME8569_4082) 4428462..4428812 + 351 WP_000239577.1 endoribonuclease toxin ChpB Toxin
ECDH1ME8569_RS21800 (ECDH1ME8569_4083) 4428892..4429422 - 531 WP_000055075.1 inorganic diphosphatase -
ECDH1ME8569_RS21805 (ECDH1ME8569_4084) 4429732..4430688 + 957 WP_000265913.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
ECDH1ME8569_RS21810 (ECDH1ME8569_4085) 4430828..4432330 + 1503 WP_000205825.1 sugar ABC transporter ATP-binding protein -
ECDH1ME8569_RS21815 (ECDH1ME8569_4086) 4432344..4433366 + 1023 WP_001313531.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12492.40 Da        Isoelectric Point: 5.5572

>T27425 WP_000239577.1 NC_017638:4428462-4428812 [Escherichia coli DH1]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T27425 NC_017638:4428462-4428812 [Escherichia coli DH1]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT27425 WP_001223208.1 NC_017638:4428217-4428468 [Escherichia coli DH1]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT27425 NC_017638:4428217-4428468 [Escherichia coli DH1]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XNP6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References