Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 46156..46954 Replicon plasmid pEC012-2
Accession NZ_CP119579
Organism Escherichia coli strain C012_chr

Toxin (Protein)


Gene name ataT Uniprot ID E2QDF3
Locus tag P1J04_RS24615 Protein ID WP_000072677.1
Coordinates 46156..46677 (-) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A0V9R6Q7
Locus tag P1J04_RS24620 Protein ID WP_001351987.1
Coordinates 46685..46954 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1J04_RS24600 (P1J04_24600) 44705..45028 + 324 WP_000064175.1 hypothetical protein -
P1J04_RS24605 (P1J04_24605) 45042..45734 + 693 WP_012640731.1 hypothetical protein -
P1J04_RS24610 (P1J04_24610) 45736..45987 + 252 WP_000901559.1 hypothetical protein -
P1J04_RS24615 (P1J04_24615) 46156..46677 - 522 WP_000072677.1 GNAT family N-acetyltransferase Toxin
P1J04_RS24620 (P1J04_24620) 46685..46954 - 270 WP_001351987.1 DUF1778 domain-containing protein Antitoxin
P1J04_RS24625 (P1J04_24625) 47273..47941 + 669 WP_000161228.1 division plane positioning ATPase MipZ -
P1J04_RS24630 (P1J04_24630) 47947..48300 + 354 WP_160378290.1 hypothetical protein -
P1J04_RS24635 (P1J04_24635) 48361..49419 - 1059 WP_001404393.1 hypothetical protein -
P1J04_RS24640 (P1J04_24640) 49709..50449 + 741 WP_001717320.1 hypothetical protein -
P1J04_RS24645 (P1J04_24645) 50493..51833 + 1341 WP_000137333.1 DnaB-like helicase C-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-55 - 1..110648 110648


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19392.17 Da        Isoelectric Point: 8.6369

>T274244 WP_000072677.1 NZ_CP119579:c46677-46156 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10082.54 Da        Isoelectric Point: 7.0155

>AT274244 WP_001351987.1 NZ_CP119579:c46954-46685 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9R6P3

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9R6Q7

References