Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 40124..41747 Replicon plasmid pEC012-1
Accession NZ_CP119578
Organism Escherichia coli strain C012_chr

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag P1J04_RS23260 Protein ID WP_000088046.1
Coordinates 40124..41449 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag P1J04_RS23265 Protein ID WP_024136327.1
Coordinates 41454..41747 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1J04_RS23230 (P1J04_23230) 36108..36965 - 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
P1J04_RS23235 (P1J04_23235) 36981..37289 - 309 WP_001224687.1 hypothetical protein -
P1J04_RS23240 (P1J04_23240) 37838..38263 - 426 WP_001043843.1 hypothetical protein -
P1J04_RS23245 (P1J04_23245) 38517..39332 - 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
P1J04_RS23250 (P1J04_23250) 39345..39755 - 411 WP_000985911.1 hypothetical protein -
P1J04_RS23255 (P1J04_23255) 39857..40063 - 207 WP_000134171.1 hypothetical protein -
P1J04_RS23260 (P1J04_23260) 40124..41449 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
P1J04_RS23265 (P1J04_23265) 41454..41747 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
P1J04_RS23270 (P1J04_23270) 42351..42743 - 393 WP_000341066.1 hypothetical protein -
P1J04_RS23275 (P1J04_23275) 43116..43457 + 342 WP_001151575.1 hypothetical protein -
P1J04_RS23280 (P1J04_23280) 43549..43740 + 192 WP_000398480.1 hypothetical protein -
P1J04_RS23285 (P1J04_23285) 43790..44071 + 282 WP_000077926.1 hypothetical protein -
P1J04_RS23290 (P1J04_23290) 44412..44543 - 132 WP_000374059.1 hypothetical protein -
P1J04_RS23295 (P1J04_23295) 44634..45875 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / tet(M) / mcr-1.1 / aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / floR / sul2 / aph(4)-Ia / aac(3)-IVa / blaCTX-M-14 / fosA3 - 1..257019 257019


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T274243 WP_000088046.1 NZ_CP119578:c41449-40124 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT274243 WP_024136327.1 NZ_CP119578:c41747-41454 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References