Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 869502..870156 Replicon chromosome
Accession NZ_CP119577
Organism Escherichia coli strain C012_chr

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag P1J04_RS04285 Protein ID WP_000244772.1
Coordinates 869749..870156 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag P1J04_RS04280 Protein ID WP_000354046.1
Coordinates 869502..869768 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1J04_RS04260 (865789) 865789..866100 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
P1J04_RS04265 (866264) 866264..866923 + 660 WP_000250274.1 hemolysin III family protein -
P1J04_RS04270 (867046) 867046..868026 - 981 WP_000399648.1 IS110-like element IS621 family transposase -
P1J04_RS04275 (868279) 868279..869259 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
P1J04_RS04280 (869502) 869502..869768 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
P1J04_RS04285 (869749) 869749..870156 + 408 WP_000244772.1 protein YgfX Toxin
P1J04_RS04290 (870196) 870196..870717 - 522 WP_001055874.1 flavodoxin FldB -
P1J04_RS04295 (870829) 870829..871725 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
P1J04_RS04300 (871750) 871750..872460 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
P1J04_RS04305 (872466) 872466..874199 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 867046..868026 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T274226 WP_000244772.1 NZ_CP119577:869749-870156 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT274226 WP_000354046.1 NZ_CP119577:869502-869768 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References