Detailed information of TA system TA274180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 917746..918396
• Replicon: chromosome
• Accession: NZ_CP119540
• Organism: Providencia stuartii strain CAVP496

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: K8W3H1
• Locus tag: P2E04_RS03910
• Protein ID: WP_004927064.1
• Coordinates: 917746..917949 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: P2E04_RS03915
• Protein ID: WP_154635730.1
• Coordinates: 918028..918396 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P2E04_RS03885 (P2E04_03885)	913626..913964	+	339	WP_154622397.1	P-II family nitrogen regulator	-
P2E04_RS03890 (P2E04_03890)	913975..915258	+	1284	WP_276123014.1	ammonium transporter AmtB	-
P2E04_RS03895 (P2E04_03895)	915486..916352	-	867	WP_276123015.1	acyl-CoA thioesterase II	-
P2E04_RS03900 (P2E04_03900)	916677..917135	+	459	WP_196713815.1	YbaY family lipoprotein	-
P2E04_RS03910 (P2E04_03910)	917746..917949	-	204	WP_004927064.1	HHA domain-containing protein	Toxin
P2E04_RS03915 (P2E04_03915)	918028..918396	-	369	WP_154635730.1	Hha toxicity modulator TomB	Antitoxin
P2E04_RS03920 (P2E04_03920)	918964..920301	-	1338	WP_154624041.1	murein transglycosylase D	-
P2E04_RS03925 (P2E04_03925)	920380..921135	-	756	WP_154624042.1	hydroxyacylglutathione hydrolase	-
P2E04_RS03930 (P2E04_03930)	921175..921900	+	726	WP_154624043.1	methyltransferase domain-containing protein	-
P2E04_RS03935 (P2E04_03935)	921967..922437	-	471	WP_154624044.1	ribonuclease HI	-
P2E04_RS03940 (P2E04_03940)	922492..923253	+	762	WP_154624045.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8075.37 Da
• Isoelectric Point: 6.9770

>T274180 WP_004927064.1 NZ_CP119540:c917949-917746 [Providencia stuartii]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSEDELELFYSAADHRLAELTMNKLYDKIPASVWKFVR

Nucleotide

• Length: 204 bp

>T274180 NZ_CP119540:c917949-917746 [Providencia stuartii]
ATGACCAAAACTGATTATCTGATGCGTTTAAGAAAATGCACAACCATCGATACACTTGAGCGTGTTATTGAAAAAAATAA
GTATGAGCTTTCTGAAGACGAGCTGGAACTATTTTACTCAGCCGCAGATCACCGTCTTGCAGAACTCACTATGAATAAAC
TTTATGACAAAATTCCAGCCTCTGTTTGGAAATTTGTTAGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14106.92 Da
• Isoelectric Point: 4.3486

>AT274180 WP_154635730.1 NZ_CP119540:c918396-918028 [Providencia stuartii]
MDEYSPKNYDISELKYLCNSLNREAISSLQKTNTHWINDLSSPQSVRLNELIEHIAAFVWQYKIKHPKDNLVISLVEEYL
DETYDLFGSPVITLSEIIDWQSMNQNLVAVLDDDLKCPASKT

Nucleotide

• Length: 369 bp

>AT274180 NZ_CP119540:c918396-918028 [Providencia stuartii]
ATGGATGAATATTCACCCAAAAATTATGATATATCAGAGTTAAAATACTTGTGTAACAGTTTGAATAGAGAAGCAATTTC
AAGCTTACAGAAAACAAATACACATTGGATAAATGATTTAAGCTCACCACAAAGTGTACGGCTCAATGAGCTTATTGAAC
ATATAGCCGCCTTTGTATGGCAATATAAGATCAAACATCCAAAAGATAATCTCGTTATATCATTAGTAGAAGAGTATCTT
GACGAGACTTACGATTTATTCGGTAGTCCGGTAATAACGTTAAGTGAAATTATTGACTGGCAGAGTATGAATCAAAATCT
TGTCGCTGTACTTGATGATGATTTAAAATGCCCTGCAAGCAAGACCTAA

Structures

Toxin

• AlphaFold DB: K8W3H1