Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3018323..3018977 Replicon chromosome
Accession NC_017638
Organism Escherichia coli DH1

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag ECDH1ME8569_RS14885 Protein ID WP_000244777.1
Coordinates 3018323..3018730 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ECDH1ME8569_RS14890 Protein ID WP_000354046.1
Coordinates 3018711..3018977 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH1ME8569_RS14865 (ECDH1ME8569_2794) 3014280..3016013 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
ECDH1ME8569_RS14870 (ECDH1ME8569_2795) 3016019..3016729 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ECDH1ME8569_RS14875 (ECDH1ME8569_2796) 3016754..3017650 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ECDH1ME8569_RS14880 (ECDH1ME8569_2797) 3017762..3018283 + 522 WP_001055874.1 flavodoxin FldB -
ECDH1ME8569_RS14885 (ECDH1ME8569_2798) 3018323..3018730 - 408 WP_000244777.1 toxin CptA Toxin
ECDH1ME8569_RS14890 (ECDH1ME8569_2799) 3018711..3018977 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ECDH1ME8569_RS14895 (ECDH1ME8569_2800) 3019220..3020200 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
ECDH1ME8569_RS14900 (ECDH1ME8569_2801) 3020396..3021055 - 660 WP_000250274.1 hemolysin III family protein -
ECDH1ME8569_RS14905 (ECDH1ME8569_2802) 3021219..3021530 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
ECDH1ME8569_RS14910 (ECDH1ME8569_2803) 3021575..3023008 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
ECDH1ME8569_RS14915 (ECDH1ME8569_2804) 3023065..3023808 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T27418 WP_000244777.1 NC_017638:c3018730-3018323 [Escherichia coli DH1]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T27418 NC_017638:c3018730-3018323 [Escherichia coli DH1]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT27418 WP_000354046.1 NC_017638:c3018977-3018711 [Escherichia coli DH1]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT27418 NC_017638:c3018977-3018711 [Escherichia coli DH1]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References