27416

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 2755024..2755691 Replicon chromosome
Accession NC_017638
Organism Escherichia coli DH1

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag ECDH1ME8569_RS13615 Protein ID WP_001094400.1
Coordinates 2755362..2755691 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag ECDH1ME8569_RS13610 Protein ID WP_000072690.1
Coordinates 2755024..2755341 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH1ME8569_RS13590 2750076..2751085 - 1010 Protein_2605 arsenic transporter -
ECDH1ME8569_RS13595 (ECDH1ME8569_2561) 2751227..2752930 + 1704 WP_000896263.1 hypothetical protein -
ECDH1ME8569_RS24070 2753502..2753725 + 224 Protein_2607 DUF905 family protein -
ECDH1ME8569_RS13600 (ECDH1ME8569_2562) 2753828..2754286 + 459 WP_000211841.1 antirestriction protein -
ECDH1ME8569_RS13605 (ECDH1ME8569_2563) 2754295..2754777 + 483 WP_001407480.1 RadC family protein -
ECDH1ME8569_RS24075 (ECDH1ME8569_2564) 2754786..2754986 + 201 WP_001395452.1 DUF987 domain-containing protein -
ECDH1ME8569_RS13610 (ECDH1ME8569_2565) 2755024..2755341 + 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
ECDH1ME8569_RS13615 (ECDH1ME8569_2566) 2755362..2755691 + 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
ECDH1ME8569_RS23015 (ECDH1ME8569_2567) 2756055..2760635 - 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T27416 WP_001094400.1 NC_017638:2755362-2755691 [Escherichia coli DH1]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T27416 NC_017638:2755362-2755691 [Escherichia coli DH1]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT27416 WP_000072690.1 NC_017638:2755024-2755341 [Escherichia coli DH1]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT27416 NC_017638:2755024-2755341 [Escherichia coli DH1]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
AlphaFold DB P52141

References