Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5222237..5222862 Replicon chromosome
Accession NZ_CP119408
Organism Klebsiella pneumoniae strain NW202109

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag P0M34_RS25490 Protein ID WP_002882817.1
Coordinates 5222237..5222620 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag P0M34_RS25495 Protein ID WP_004150355.1
Coordinates 5222620..5222862 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P0M34_RS25475 (5219602) 5219602..5220504 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
P0M34_RS25480 (5220501) 5220501..5221136 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
P0M34_RS25485 (5221133) 5221133..5222077 + 945 WP_280991071.1 formate dehydrogenase accessory protein FdhE -
P0M34_RS25490 (5222237) 5222237..5222620 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
P0M34_RS25495 (5222620) 5222620..5222862 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
P0M34_RS25500 (5223067) 5223067..5223984 + 918 WP_021466676.1 alpha/beta hydrolase -
P0M34_RS25505 (5223998) 5223998..5224939 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
P0M34_RS25510 (5224984) 5224984..5225421 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
P0M34_RS25515 (5225418) 5225418..5226278 - 861 WP_004146232.1 virulence factor BrkB family protein -
P0M34_RS25520 (5226272) 5226272..5226871 - 600 WP_004146231.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T274124 WP_002882817.1 NZ_CP119408:c5222620-5222237 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT274124 WP_004150355.1 NZ_CP119408:c5222862-5222620 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References