Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4217786..4218283 Replicon chromosome
Accession NZ_CP119404
Organism Escherichia coli strain MRE162 substr. UK

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PX811_RS20625 Protein ID WP_001173343.1
Coordinates 4217786..4217959 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag PX811_RS20630 Protein ID WP_000398619.1
Coordinates 4217987..4218283 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PX811_RS20615 (4214456) 4214456..4215913 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
PX811_RS20620 (4216150) 4216150..4217667 + 1518 WP_001295074.1 lysine--tRNA ligase -
PX811_RS20625 (4217786) 4217786..4217959 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PX811_RS20630 (4217987) 4217987..4218283 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PX811_RS20635 (4218510) 4218510..4218782 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
PX811_RS20640 (4218794) 4218794..4219024 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
PX811_RS20645 (4219205) 4219205..4220836 + 1632 WP_001216477.1 sensor histidine kinase -
PX811_RS20650 (4220833) 4220833..4221552 + 720 WP_275771340.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T274111 WP_001173343.1 NZ_CP119404:c4217959-4217786 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT274111 WP_000398619.1 NZ_CP119404:c4218283-4217987 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References