Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4212457..4212954 Replicon chromosome
Accession NZ_CP119402
Organism Escherichia coli strain MRE162 substr. FRA

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PX810_RS20590 Protein ID WP_001173343.1
Coordinates 4212457..4212630 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag PX810_RS20595 Protein ID WP_000398619.1
Coordinates 4212658..4212954 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PX810_RS20580 (4209127) 4209127..4210584 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
PX810_RS20585 (4210821) 4210821..4212338 + 1518 WP_001295074.1 lysine--tRNA ligase -
PX810_RS20590 (4212457) 4212457..4212630 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PX810_RS20595 (4212658) 4212658..4212954 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PX810_RS20600 (4213181) 4213181..4213453 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
PX810_RS20605 (4213465) 4213465..4213695 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
PX810_RS20610 (4213876) 4213876..4215507 + 1632 WP_001216477.1 sensor histidine kinase -
PX810_RS20615 (4215504) 4215504..4216223 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T274092 WP_001173343.1 NZ_CP119402:c4212630-4212457 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT274092 WP_000398619.1 NZ_CP119402:c4212954-4212658 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References