Detailed information of TA system TA027402

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1491468..1492106
• Replicon: chromosome
• Accession: NC_017638
• Organism: Escherichia coli DH1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ECDH1ME8569_RS07390
• Protein ID: WP_000813794.1
• Coordinates: 1491468..1491644 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ECDH1ME8569_RS07395
• Protein ID: WP_001270286.1
• Coordinates: 1491690..1492106 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECDH1ME8569_RS07370 (ECDH1ME8569_1376)	1487087..1488301	-	1215	WP_001326689.1	BenE family transporter YdcO	-
ECDH1ME8569_RS07375 (ECDH1ME8569_1377)	1488354..1488890	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
ECDH1ME8569_RS07380 (ECDH1ME8569_1378)	1488963..1490924	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ECDH1ME8569_RS07385 (ECDH1ME8569_1379)	1491016..1491246	-	231	WP_000494244.1	YncJ family protein	-
ECDH1ME8569_RS07390 (ECDH1ME8569_1380)	1491468..1491644	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ECDH1ME8569_RS07395 (ECDH1ME8569_1381)	1491690..1492106	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ECDH1ME8569_RS07400 (ECDH1ME8569_1382)	1492185..1493591	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
ECDH1ME8569_RS07405 (ECDH1ME8569_1383)	1493836..1494981	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
ECDH1ME8569_RS07410 (ECDH1ME8569_1384)	1494999..1496012	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
ECDH1ME8569_RS07415 (ECDH1ME8569_1385)	1496013..1496954	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T27402 WP_000813794.1 NC_017638:1491468-1491644 [Escherichia coli DH1]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T27402 NC_017638:1491468-1491644 [Escherichia coli DH1]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT27402 WP_001270286.1 NC_017638:1491690-1492106 [Escherichia coli DH1]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT27402 NC_017638:1491690-1492106 [Escherichia coli DH1]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB