27391

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 480514..481132 Replicon chromosome
Accession NC_017638
Organism Escherichia coli DH1

Toxin (Protein)


Gene name Hha Uniprot ID -
Locus tag ECDH1ME8569_RS02370 Protein ID WP_001290581.1
Coordinates 480514..480732 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ECDH1ME8569_RS02375 Protein ID WP_000344800.1
Coordinates 480758..481132 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH1ME8569_RS02340 (ECDH1ME8569_0438) 475803..476375 + 573 WP_000779842.1 YbaY family lipoprotein -
ECDH1ME8569_RS02345 (ECDH1ME8569_0439) 476406..476717 - 312 WP_000409911.1 MGMT family protein -
ECDH1ME8569_RS02350 (ECDH1ME8569_0440) 477096..477449 + 354 WP_000878140.1 DUF1428 domain-containing protein -
ECDH1ME8569_RS02355 (ECDH1ME8569_0441) 477491..479041 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ECDH1ME8569_RS02360 (ECDH1ME8569_0442) 479205..479675 - 471 WP_000136192.1 YlaC family protein -
ECDH1ME8569_RS02365 (ECDH1ME8569_0443) 479791..480342 - 552 WP_000102564.1 maltose O-acetyltransferase -
ECDH1ME8569_RS02370 (ECDH1ME8569_0444) 480514..480732 - 219 WP_001290581.1 hemolysin expression modulator Hha Toxin
ECDH1ME8569_RS02375 (ECDH1ME8569_0445) 480758..481132 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ECDH1ME8569_RS02380 (ECDH1ME8569_0446) 481678..484827 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
ECDH1ME8569_RS02385 (ECDH1ME8569_0447) 484850..486043 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8678.06 Da        Isoelectric Point: 8.9008

>T27391 WP_001290581.1 NC_017638:c480732-480514 [Escherichia coli DH1]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T27391 NC_017638:c480732-480514 [Escherichia coli DH1]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT27391 WP_000344800.1 NC_017638:c481132-480758 [Escherichia coli DH1]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT27391 NC_017638:c481132-480758 [Escherichia coli DH1]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References