Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 262553..263232 | Replicon | chromosome |
| Accession | NC_017638 | ||
| Organism | Escherichia coli DH1 | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | P77692 |
| Locus tag | ECDH1ME8569_RS01250 | Protein ID | WP_000854672.1 |
| Coordinates | 262553..262894 (-) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | Q47684 |
| Locus tag | ECDH1ME8569_RS01255 | Protein ID | WP_000070395.1 |
| Coordinates | 262915..263232 (-) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ECDH1ME8569_RS01225 (ECDH1ME8569_0228) | 257830..258231 | + | 402 | WP_000174689.1 | sigma factor-binding protein Crl | - |
| ECDH1ME8569_RS01230 (ECDH1ME8569_0229) | 258270..259325 | - | 1056 | WP_000749863.1 | phosphoporin PhoE | - |
| ECDH1ME8569_RS01235 (ECDH1ME8569_0230) | 259613..260716 | + | 1104 | WP_001285288.1 | glutamate 5-kinase | - |
| ECDH1ME8569_RS01240 (ECDH1ME8569_0231) | 260728..261981 | + | 1254 | WP_000893278.1 | glutamate-5-semialdehyde dehydrogenase | - |
| ECDH1ME8569_RS01250 (ECDH1ME8569_0232) | 262553..262894 | - | 342 | WP_000854672.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| ECDH1ME8569_RS01255 (ECDH1ME8569_0233) | 262915..263232 | - | 318 | WP_000070395.1 | type IV toxin-antitoxin system antitoxin YafW | Antitoxin |
| ECDH1ME8569_RS23105 (ECDH1ME8569_0234) | 263251..263472 | - | 222 | WP_000691994.1 | DUF987 domain-containing protein | - |
| ECDH1ME8569_RS01260 (ECDH1ME8569_0235) | 263481..263957 | - | 477 | WP_000811693.1 | RadC family protein | - |
| ECDH1ME8569_RS01265 (ECDH1ME8569_0236) | 263973..264431 | - | 459 | WP_000211838.1 | antirestriction protein | - |
| ECDH1ME8569_RS01270 (ECDH1ME8569_0237) | 264529..264768 | - | 240 | WP_000194654.1 | DUF905 domain-containing protein | - |
| ECDH1ME8569_RS01275 (ECDH1ME8569_0238) | 264845..265312 | - | 468 | WP_001547765.1 | hypothetical protein | - |
| ECDH1ME8569_RS24720 | 265335..265778 | - | 444 | WP_000824223.1 | hypothetical protein | - |
| ECDH1ME8569_RS24725 | 265778..266005 | - | 228 | WP_001548158.1 | hypothetical protein | - |
| ECDH1ME8569_RS24730 | 266001..266192 | - | 192 | Protein_248 | DeoR family transcriptional regulator | - |
| ECDH1ME8569_RS01290 (ECDH1ME8569_0240) | 266409..267230 | - | 822 | WP_000197389.1 | DUF945 domain-containing protein | - |
| ECDH1ME8569_RS01295 (ECDH1ME8569_0241) | 267322..268185 | - | 864 | WP_001065553.1 | GTPase family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12905.01 Da Isoelectric Point: 9.6543
>T27390 WP_000854672.1 NC_017638:c262894-262553 [Escherichia coli DH1]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T27390 NC_017638:c262894-262553 [Escherichia coli DH1]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11939.59 Da Isoelectric Point: 6.7391
>AT27390 WP_000070395.1 NC_017638:c263232-262915 [Escherichia coli DH1]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT27390 NC_017638:c263232-262915 [Escherichia coli DH1]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|