Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4837885..4838501 Replicon chromosome
Accession NZ_CP119165
Organism Citrobacter freundii strain 2075

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag P0S03_RS23565 Protein ID WP_003028682.1
Coordinates 4837885..4838259 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag P0S03_RS23570 Protein ID WP_043018956.1
Coordinates 4838259..4838501 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P0S03_RS23550 (4835388) 4835388..4836290 + 903 WP_003847898.1 formate dehydrogenase O subunit beta -
P0S03_RS23555 (4836287) 4836287..4836922 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
P0S03_RS23560 (4836919) 4836919..4837848 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
P0S03_RS23565 (4837885) 4837885..4838259 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
P0S03_RS23570 (4838259) 4838259..4838501 - 243 WP_043018956.1 CopG family transcriptional regulator Antitoxin
P0S03_RS23575 (4838706) 4838706..4839614 + 909 WP_172750447.1 alpha/beta hydrolase -
P0S03_RS23580 (4839765) 4839765..4840706 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
P0S03_RS23585 (4840751) 4840751..4841188 - 438 WP_003028671.1 D-aminoacyl-tRNA deacylase -
P0S03_RS23590 (4841185) 4841185..4842057 - 873 WP_003028669.1 virulence factor BrkB family protein -
P0S03_RS23595 (4842051) 4842051..4842650 - 600 WP_016151258.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T273882 WP_003028682.1 NZ_CP119165:c4838259-4837885 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT273882 WP_043018956.1 NZ_CP119165:c4838501-4838259 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References